Stage 4

 

Anolis sagrei stage 4 - head - 3D model by SangerLab (@SangerLab) [6e3d271]

Fronto-nasal process is prominent. Maxillary process is large. Lens of eye beginning to differentiate.

Incubated at 27°C Stained with 1.5% PTA Imaged in Perkin-Elmer 6x2 Processed in 3D Slicer Voxel Size: - Anolis sagrei embryo - stage 4 - 3D model by SangerLab (@SangerLab) [2dfc0f1]

Both forelimb bud and hindlimb bud are noticeably large; approximately twice as long as they are wide.

Anolis sagrei stage 4 - brain - 3D model by SangerLab (@SangerLab) [2443f6f]

At stage 4, the developing cephalic bulges are distinct enough to distinguish into the cerebrum, optic tectum, and hindbrain. The cerebellum is less distinct.